球体
脐静脉
微流控
细胞生物学
组织工程
血管网
血管组织
生物医学工程
灌注
化学
体外
解剖
生物
材料科学
纳米技术
医学
生物化学
心脏病学
植物
作者
Yuji Nashimoto,Tomoya Hayashi,Itsuki Kunita,Akiko Nakamasu,Yu-suke Torisawa,Masamune Nakayama,Hisako Takigawa-Imamura,Hidetoshi Kotera,Koichi Nishiyama,Takashi Miura,Ryuji Yokokawa
摘要
Creating vascular networks in tissues is crucial for tissue engineering. Although recent studies have demonstrated the formation of vessel-like structures in a tissue model, long-term culture is still challenging due to the lack of active perfusion in vascular networks. Here, we present a method to create a three-dimensional cellular spheroid with a perfusable vascular network in a microfluidic device. By the definition of the cellular interaction between human lung fibroblasts (hLFs) in a spheroid and human umbilical vein endothelial cells (HUVECs) in microchannels, angiogenic sprouts were induced from microchannels toward the spheroid; the sprouts reached the vessel-like structures in a spheroid to form a continuous lumen. We demonstrated that the vascular network could administer biological substances to the interior of the spheroid. As cell density in the spheroid is similar to that of a tissue, the perfusable vasculature model opens up new possibilities for a long-term tissue culture in vitro.
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