熊去氧胆酸
生物催化
生物过程
酶
化学
产量(工程)
连续反应器
辅因子
生物反应器
组合化学
制浆造纸工业
色谱法
有机化学
生物化学
催化作用
化学工程
材料科学
工程类
离子液体
冶金
作者
Haipeng Li,Bing-Yi Yang,Ying Su,Zhi‐Neng You,Wu-Tong Yin,Chun-Xiu Li,Gao‐Wei Zheng,Jingping Qü,Jian‐He Xu
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2022-12-02
卷期号:10 (50): 16916-16923
被引量:2
标识
DOI:10.1021/acssuschemeng.2c05741
摘要
Biocatalysis in continuous flow systems is an appealing strategy for efficient and environmentally sustainable manufacturing of value-added compounds. Here, a robust, productive, and sustainable closed-loop packed bed reactor system was developed for continuous asymmetric reduction of 7-oxo-lithocholic acid to ursodeoxycholic acid (UDCA). UDCA is a clinically valuable active pharmaceutical ingredient for treatment of hepatobiliary-related diseases. In this packed bed reactor, a conversion of 92% was achieved at a residence time of only 5 min, which represented an extremely high space-time yield of 1040 gUDCA L–1 d–1. Furthermore, the reactor exhibited excellent operational stability, maintaining a steady conversion above 95% after 31 days of continuous operation with a residence time of 10 min. By recovering and recycling the used reaction buffer containing the expensive cofactor (NAD+), the environment factor of this bioprocess was greatly reduced from 293 to 77, and the cost for UDCA biosynthesis decreased by approximately 21%. This closed-loop enzymatic system improves the potential for application of environmentally benign and cost-effective operation modes for bioprocesses that require expensive cofactors.
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