生物标志物
荧光
化学
半胱氨酸
色谱法
生物化学
物理
量子力学
酶
作者
Sheng Wang,Jun Xu,F.-R. Yue,Lina Zhang,Ning Bi,Jian Gou,Yongxin Li,Yuanyuan Huang,Ting Zhao,Lei Jia
标识
DOI:10.1016/j.foodchem.2024.139410
摘要
Dipicolinic acid (DPA), as a biomarker for Bacillus anthracis, is highly toxic at trace levels. Rapid and on-site quantitative detection of DPA is essential for maintaining food safety and public health. This work develops a dual-channel self-calibrated fluorescence sensor constructed by the YVO4:Eu and Tb-β-diketone complex for rapid visual detection of DPA. This sensor exhibits high selectivity, fast response time, excellent detection sensitivity, and the detection limit is as low as 4.5 nM in the linear range of 0-16 μM. A smartphone APP and portable ultraviolet lamp can assemble a mobile fluorescence sensor for on-site analysis. Interestingly, adding Cu2+ ions can quench the fluorescence intensity of Tb3+. In contrast, the addition of cysteine can restore the fluorescence, allowing the accurate detection of Cu2+ ions and cysteine in environmental water and food samples. This work provides a portable sensor that facilitates real-time analysis of multiple targets in food and the environment.
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