Preparation and anti-tumor effect of quercetin nano-liposome

Objective To prepare quercetin liposome and to explore the antitumor effect of quercetin liposome. Methods The cholesterol and lecithin were used as membrane materials, quercetin nano liposome was prepared by thin film ultrasound method.The zeta potential and particle size distribution of quercetin liposome were tested by Malvern laser particle size analyzer and transmission electron microscope respectively.In order to explore the anti-tumor effect of quercetin nano-liposome, the mouse model of cervical cancer was established.After tail vein injection of quercetin and quercetin nano-liposome for 15 days, the tumor inhibitory rate, the thymus (spleen) index were analyzed, and the pathology of tumor tissues was further observed. Results Under the condition of lecithin: cholesterol: quercetin=8∶2∶1, the hydration time of 15 min and the ultrasonic time of 15 min, the quercetin nano-liposome was prepared, and the particle size distribution was uniform and the potential was -10.8.The tumor inhibitory rate of quercetin nano-liposome treatment group was 54.16%, which was significantly higher than that of the quercetin treatment group (χ2=6.477, P<0.05). The pathology results of the tumor tissues showed that nanocrystallization of quercetin could increase the anti-cancer effect of quercetin. Conclusion Both quercetin and quercetin nano-liposome exhibit significant effect on the tumor growth, and the inhibitory rate is increased after quercetin was nanocrystallization.Our study will provide theoretical basis for the application of quercetin nano-liposome in the treatment of cervical cancer. Key words: Quercetin; Nano liposome; Thin film ultrasonic method; Antitumor effect