A new kind of mistuning—pre-tightening mistuning was found in the bladed disk based on the engineering practice.The characteristics of bladed disk with pre-tightening mistuning and stiffness mistuning was compared and analyzed based on a continuous parameter model of bladed disk,and the effect of pre-tightening mistuning on the vibration response localization of bladed disk was researched.The result shows that pre-tightening mistuning can make vibration response localization appear in bladed disk as well as stiffness mistuning.Sometimes,the vibration response localization of bladed disk is more sensitive to stiffness mistuning than to pre-tightening mistuning.Compared with stiffness mistuning,the pre-tightening mistuning could not change the natural characteristics of bladed disk.As a result,the pre-tightening mistuning can not be detected by traditional method,such as the frequency test.The result could not only complete the study content of bladed disk,but also conduct the design and manufacture of bladed disk.