Effects of Saikosaponin d on ERK1/2 Expression and Cell Apoptosis in SH-SY5Y Cells

Objective To investigate the effects of Saikosaponin d(SSd) on p-ERKl/2 expression and apoptosis in human neuroblastoma cells(SH-SY5Y).Methods SH-SY5Y cell lines were established in vitro firstly,then SSd was dissolved in high glucose DMEM cultures which including 0.3%DMSO.The cultures were individually pretreated with 0-10 μmol/L SSd for 48 hours,then the expressions of t-ERKl/2 and p-ERKl/2were analyzed by western-blotting.Meanwhile,cell apoptosis levels were analyzed by Hoechst33342 and flow cytometry.Results A significant reduciton of p-ERKl/2 was observed in SH-SY5Y cells after 48 h treatment of SSd at concentrations between 8—10 μmol/L.It was also shown that SSd from 8-10 μmol/L groups could induced cell apoptosis of SH-SY5Y cells.Conclusion These results demonstrate that SSd can promote the apoptosis of SH-SY5Y cells,and the mechanism may be related to the inhibition of ERK1/2 active expression.