Establishment of Rapid and Simple Primary Screening of Enterococcus

Objective To study and apply a rapid and simple screening method of Enterococcus.Methods A total of 139 strains of the gram-positive coccus were investigated for esculin hydrolysis by three methods of esculin rapid hydrolysis kit,conventional methods and API 20 Strep system(bioMerieux).Results The positive rate of fluorescence loss was 99.26% in Enterococcus, 100% in Enterococcus faecalis,and 91.66% in Enterococcus faecium.The positive rate of forming light gray or black spots was 99.26%,of which Enterococcus faecalis was 100%,Enterococcus faecium for 91.66 %.Conclusion Through the methods of esculin hydrolysis,Gram's method,microscopic morphology,catalase test and colony characteristics on selective and differential medium,it can be rapidly and simply identified for Enterococcus at the genus level.