HPLC Analysis on Formation of the Multi-Bioinsecticidal-Toxin BtA

Based on the theory of multi-bioinsecticidal toxin and the technique of bioconjugation,The multi-bioinsecticidal-toxin BtA was worked out in order to provide a new idea and a new method for development of a new type of biocide. The bioconjugation procedure of BtA included: decomposing Bacillus thuringiensis (B.t.) crystal by DTT into the protoxin with an end amino group; activation and derivation of abamectin to form abamectin-COONa with a carboxylic group; final bioconjugation by using amino-carboxylic conjugator EDC to achieve the substance combination of two biotoxins. In the detecting program,the experiments were carried out with HPLC (High Pressure Liquid Chromatogram) on measuring BtA bioconjugation system at different reaction times to identify the occurrence of bioconjugation reaction,on comparing BtA bioconjugate system with the three reactants and three two-reactant combinations to distinguish bioconjugated product BtA,and on a qualitative analysis of the bioconjugated product BtA. The results proved that a new bioconjugated substance was confirmed to present in BtA bioconjugation system,which was named as multi-bioinsecticidal-toxin BtA.