内皮干细胞
干细胞
造血
血管生成
再生(生物学)
诱导多能干细胞
血管生成
作者
Susumu Hara,Ryuhei Hayashi,Takeshi Soma,Tomofumi Kageyama,Thomas Duncan,Motokazu Tsujikawa,Kohji Nishida
出处
期刊:Stem Cells and Development
[Mary Ann Liebert]
日期:2014-03-03
卷期号:23 (18): 2190-2201
被引量:49
标识
DOI:10.1089/scd.2013.0387
摘要
The corneal endothelium is believed to be developmentally originated from the periocular mesenchyme via the neural crest. Human corneal endothelial progenitor cells (HCEPs) have been investigated because of their potential availability for the tissue regenerative medicine. However, the existence and the properties of HCEPs have not been elucidated yet. We first established a novel serum-free culture system for HCEPs. The HCEPs highly expressed p75 neurotrophin receptor, SOX9, and FOXC2, and partially retained the properties of neural crest and periocular mesenchyme. Further, we demonstrated that HCEPs had a high proliferative potency, and the differentiated HCEP sheets had corneal endothelial function by using the Ussing chamber system and transplantation to the rabbit cornea. These findings suggest that the HCEPs can be selectively expanded from the corneal endothelium using a specific culture system and will provide cell sheets for corneal regenerative medicine.
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