活性氧
绿萍
光系统
析氧
光合作用
700页
化学
电子传输链
叶绿素荧光
光系统II
光系统I
叶绿体
生物物理学
光合反应中心
类囊体
叶绿素
质体蓝素
植物
生物化学
生物
水生植物
基因
生态学
电极
物理化学
水生植物
电化学
作者
Abdallah Oukarroum,Filippo Bussotti,Vasilij Goltsev,Hazem M. Kalaji
标识
DOI:10.1016/j.envexpbot.2014.08.005
摘要
Increased rate of reactive oxygen species (ROS) is a common plant response to various environmental stresses. In chloroplast thylakoids, the reaction centers of photosystems I and II are the major generation site of ROS. In the present study, the changes of chlorophyll a fluorescence parameters, P700 absorption change and ROS production (using the fluorescent probe 2,7-dichlorofluorescin diacetate) were investigated in Lemna gibba plants exposed to salt stress (0–400 mM NaCl). Salt stress inhibited PSI and PSII activities and resulted in a decrease in overall activity of the electron transport chain while stimulating ROS production. When L. gibba plants were kept into dark condition, NaCl treatment did not showed any significant change in ROS formation compared to control. However, NaCl treatment in light condition induced a strong increase in ROS formation. The production of ROS at 400 mM NaCl was 2.6 and 10 fold higher compared to the control respectively after 6 h and 24 h treatment in light. Furthermore, the correlation between ROS production and the two photosystems (PSI and PSII) activities in L. gibba plants was analyzed. Our data confirmed the correlation between the ROS production and PSII and PSI activities. We showed that ROS production was highly correlated to maximal quantum yield of PSII (R2 = 0.91) and efficiency with which a trapped exciton can move an electron into the electron transport chain (R2 = 0.86). While the correlation coefficient (R2) value ROS formation and ΔI/Io 820 nm (measure of redox states of plastocyanin and P700) was 0.63.
科研通智能强力驱动
Strongly Powered by AbleSci AI