姜黄素
壳聚糖
动态光散射
Zeta电位
纳米颗粒
右旋糖酐
核化学
化学
傅里叶变换红外光谱
MTT法
毒品携带者
药物输送
荧光显微镜
体外
荧光
色谱法
化学工程
材料科学
纳米技术
生物化学
有机化学
物理
量子力学
工程类
作者
A Anitha,V. G. Deepagan,Vibha Rani,Deepthy Menon,Shantikumar V. Nair,R. Jayakumar
标识
DOI:10.1016/j.carbpol.2011.01.005
摘要
Nanoformulation of curcumin, (a low molecular weight hydrophobic drug) was prepared by using dextran sulphate and chitosan. The developed nanoparticles were characterized by Dynamic Light Scattering measurements (DLS), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD) and Differential Thermal Analysis (DTA). The prepared system showed an average size of 200–220 nm with a zeta potential value of −30 mV and showed ∼74% drug entrapment efficiency. In vitro drug release studies showed a controlled and pH dependent curcumin release over a period of one week. The cytocompatibility of bare nanoparticles was verified by MTT assay; cellular internalisation of curcumin loaded nanoparticles was confirmed by fluorescent imaging and quantified spectrophotometrically, anticancer activity of curcumin loaded nanoparticles was proved by MTT assay and reconfirmed by apoptosis assay (FACS). The results showed preferential killing of cancer cells compared to normal cells by the curcumin-loaded nanoparticles. Thus the developed curcumin loaded nanoformulation could be a promising candidate in cancer therapy.
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