With new varieties of the Lilium longiflorum Thunb ShenXiang1 as the experimental material,determined and analyzed the effects of hormones on physiological and biochemical variations of the Lilium longiflorum Thunb,on MS medium added 6-BA 0.5 mg/L+NAA 0.1 mg/L and one without hormone.The results showed that with the hormome treatment of regenerated seedings of the Lilium longiflorum Thunb,the increasing of soluble protein and non-soluble protein content was much bigger.Proper hormome treatment could induce the mutually transformational of soluble sugar and starch content,could induce Malondidehyde(MDA) content and bring down superoxide dismutase(SOD) of the Lilium longiflorum Thunb.In conclusion,hormone treatment could enhance the protein and carbohydrate metabolism in the process of the Lilium longiflorum Thunb differentiation and de-differentiation,and improved organizational resistance.