An Optimized Method for Suspension Culture of CHO Cells to Produce Recombinant Human Erythropoietin(EPO)

Objective: To screen and domesticate the adherent cultured CHO cells to obtain high expression of cell suspension culture for production of recombinant human erythropoietin erythropoietin(rHuEPO).Methods: Using 96-well and 24-well plates culture method to screen and domesticate the highly expressing CHO cell strain.Acclimate the high expression cell strain and make it suitable for suspension culture.It's inoculated into the bioreactor in serum-free culture after amplified by the shake flask,and monitoring of glucose content,measuring rHuEPO expression of daily.Results: The suspension culture of CHO cell production of rHuEPO has short production period,higher expression than adherent culture.On the other hand,it is easy to operate and scale-up,but not easy to pollute.Furthermore,we established of the CHO cell strain for suspension culture,which provided a technical basis for industrial production of CHO cells the rHuEPO.Conclusion: After process optimization,the use of serum-free suspension culture production of erythropoietin average expression has high,short production period,low cost of production.than adherent culture.