Effect of Zishen Jiangtang Pills on Adipogenic Differentiation of Mice MSC and Its Mechanisms

OBJECTIVE:To investigate the effect of Zishen jiangtang pills on adipogenic differentiation of mice mesenchymal stem cells (MSC) and its mechanisms.METHODS:Mice were given Zishen jiangtang pill via i.g.gtt at a dose of 1.5 g·kg-1 once a day for a continuous week.Serum sample was collected 2 h after last administration.Then,effects of serum containing active components on adipogenic differentiation of MSC were investigated by means of oil red O staining.The expressions of PPARγ2 and FABP4 were determined by real-time Q-RT-PCR method during adipogenic differentiation of MSC.RESULTS:The serum containing active components inhibited the adipogenic differentiation of MSC significantly.The expressions of PPARγ2 and FABP4 were significantly decreased after 3 and 6 days of adipogenesis induction.CONCLUSION:Zishen jiangtang pills can inhibit adipogenic differentiation of MSC,which might related to the expressions inhibition of PPARγ2 and FABP4.