High-mobility group box protein1 increases tumor necrosis factor α expression in human bronchial epithelial cells

Objective To investigate the effect of high-mobility group box protein1(HMGB1) on the expression of TNF-α and its mechanism in 16 HBE in vitro. Methods groups with different HMGB1(0, 100, 500,2 000 ng / m L) concentration was set; RAGE antagonizing groups were as control, HMGB1-2000 ng, anti-RAGE and anti-RAGE+HMGB1. The changes of TNF-α mRNA and secretion were determined by quantitative PCR and ELISA.RAGE protein level was measured by western blotting. Results HMGB1 intervention and TNF-α expression of16 HBE presented a positive dose-dependent relationship. Thechanges of RAGE was HMGB1 positively concentration dependent. In comparison with HMGB1 2 000 ng / m L group, anti-RAGE+HMGB1showed a remarkable reduction of TNF-α secretion. Conclusion In vitro, HMGB1 increases TNF-α expression in 16 HBE with a dose-dependent manner through RAGE.