Correlation of tissue infection and serologic findings in pigs fed Toxoplasma gondii oocysts

SUMMARY Each of thirteen 6-week-old pigs was inoculated per os with 10,000 sporulated oocysts of Toxoplasma gondii . By postinoculation day ( pid ) 13, pigs were seropositive by the indirect fluorescent antibody test. Beginning on pid 13 and every 7 days thereafter through pid 97, 1 pig was killed and 6 tissues were examined for T gondii . Of the 13 pigs, 11 were infected, including the 1st pig killed on pid 13, although none of the pigs had gross lesions of toxoplasmosis. Tissues harboring T gondii most frequently were the heart and brain; organisms were detected less frequently in the longissimus muscles, diaphragm, and liver. Toxoplasma gondii was not detected in the bronchial lymph nodes. There was good correlation between antibody and presence of T gondii in these pigs. One additional pig, maintained as a noninfected control, remained seronegative and had no evidence of infection when killed on pid 97.