Study on cotyledon and hypocotyl plant regeneration of processing tomato in vitro_

Cotyledons and hypocotyls of BO2 and BO4 process tomatoes were used as explants to study the effects of different concentrations of ZT,6-BA combined with different concentrations of IAA on bud induction.The results showed that the best shoot induction medium for cotyledon and hypocotyls explants was MS+ZT 1.0 mg/L+IAA 0.05 mg/L in different combinations of ZT and IAA.The shoot induction ratios were 32.7% and 24.5% for BO2 cotyledon and hypocotyls explants and 37.3% and 28.2% for BO4 cotyledon and hypocotyls explants respectively.Among different combinations of 6-BA and IAA,the best medium for BO2 was MS+6-BA 2.0 mg/L+IAA 0.2 mg/L and the shoot induction ratio was 27.3% and 14.5% for cotyledon and hypocotyls explants respectively.The most suitable induction medium for cotyledon and hypocotyls explants of BO4 was MS+6-BA 1.0 mg/L+IAA 0.2 mg/L and MS+6-BA 2.0 mg/L+IAA 0.2 mg/L respectively and the shoot induction ratio was 28.2%.The root could be induced after the regenerated buds were planted in MS+IAA 0.1 mg/L one week later.