电化学发光
鲁米诺
化学
检出限
沸石咪唑盐骨架
量子点
三元运算
咪唑酯
生物分子
生物传感器
纳米棒
纳米技术
组合化学
无机化学
金属有机骨架
色谱法
材料科学
有机化学
程序设计语言
生物化学
吸附
计算机科学
作者
Wei Liu,Meiling Su,Anyi Chen,Kanfu Peng,Yaqin Chai,Ruo Yuan
标识
DOI:10.1021/acs.analchem.2c01444
摘要
Herein, a highly efficient electrochemiluminescence (ECL) emitter, luminol/MoS2 quantum dots@zeolitic imidazolate framework-8 (Lu/MoS2 QDs@ZIF-8), with a positive charge was prepared to construct a novel luminol-H2O2-MoS2 QD ternary ECL system for ultrasensitive detection of microRNA-21 (miRNA-21). The porous Lu/MoS2 QDs@ZIF-8 was beneficial for reducing the accessible distance between various participants in the ternary system wherein co-reaction accelerator MoS2 QDs promoted H2O2 to generate superoxide anion radicals (O2•-), which instantaneously reacted with luminol to produce robust ECL signals. Simultaneously, the positively charged Lu/MoS2 QDs@ZIF-8 facilitated the enrichment of O2•- to further improve the ECL efficiency of luminol. Impressively, compared with the traditional binary luminol-H2O2 system, the ECL efficiency of this ternary system was increased by 12.7 times. In the aid of a target-cycled and endogenous adenosine triphosphate-driven signal amplification strategy, the biosensor with Lu/MoS2 QDs@ZIF-8 as an ECL emitter achieved ultrasensitive detection for miRNA-21 with a detection limit of 14.6 aM. This work provides a promising perspective to construct a highly efficient ECL ternary system for biomolecule detection and potential disease diagnosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI