Quantitative OCT analysis of sperm hyperactivation state in mouse fallopian tube

Spermatozoa motility is critical for successfully reaching the egg and fertilization. One of essential transitions in sperm behavior within the female reproductive tract required for fertilization is hyperactivation. Our group established an innovative volumetric in vivo sperm tracking approach using OCT through an intravital imaging window. This study is focused on the development of new functional OCT quantitative measures for the analysis of sperm hyperactivation state. The quantitative parameters were first developed in vitro; then optimized and tested in vivo. This work established a potential quantitative approach for differentiating sperm hyperactivation status based on their trajectories in vivo.