WRKY蛋白质结构域
石碑
突变体
抑制因子
生物
基因
野生型
分子生物学
转录因子
拟南芥
生物化学
植物
作者
Zhong Jie Ding,Jun Yan,Xiao Yan Xu,Gui Xin Li,Shao Jian Zheng
出处
期刊:Plant Journal
[Wiley]
日期:2013-11-05
卷期号:76 (5): 825-835
被引量:154
摘要
Summary Aluminum ( A l) toxicity is the major limiting factor for crop production on acid soils, but the transcriptional regulation of A l tolerance genes is largely unknown. Here, we found that the expression of a WRKY domain‐containing transcription factor WRKY 46 is inhibited by A l and expressed in root stele, whereas the expression of ALMT 1 , which encodes a malate efflux transporter, is induced by A l stress and spatially co‐localized with WRKY 46 in root stele, indicating the possible interaction between WRKY 46 and ALMT 1 in A rabidopsis. Mutation of WRKY4 6 by T–DNA insertion leads to better root growth under A l stress, and lower root A l content compared with the wild‐type Col–0. The wrky46 mutant shows increased root malate secretion, which is consistent with the higher ALMT 1 expression in the mutant. Transient expression analysis using truncated promoter of ALMT 1 showed that ALMT 1 expression can be inhibited by WRKY 46 in tobacco leaves. The yeast one‐hybrid assay and C h IP ‐q PCR analysis revealed that WRKY 46 directly binds to ALMT1 promoter through specific W –boxes. Taken together, we demonstrated that WRKY46 is a negative regulator of ALMT1 , mutation of WRKY 46 leads to increased malate secretion and reduced Al accumulation in root apices, and thus confers higher A l resistance.
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