Assignment of the NAD-dependent deacetylase sirtuin 5 gene <i>(SIRT5)</i> to human chromosome band 6p23 by in situ hybridization

Sirtuin 5 (SIRT5) is a nicotinamide adenine dinucleotide (NAD⁺)-dependent deacetylase and belongs to the Silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (HDACs), which play a central role in epigenetic gene silencing, DNA repair and recombination, cell-cycle, microtubule organization, and in the regulation of aging. We have isolated and characterized the human <i>SIRT5</i> genomic sequence, which spans a region of 28,182 bp and which has one single genomic locus. Human <i>SIRT5</i> consists of eight exons and is found in two isoforms, which encode a 310 aa and a 299 aa protein, respectively. Human <i>SIRT5</i> is most predominantly expressed in heart muscle cells and in lymphoblasts. Fluorescence in situ hybridization analysis localized the human <i>SIRT5</i> gene to chromosome 6p23.