Directed enzyme evolution : screening and selection methods

Part I. Genetic Selections Genetic Complementation Protocols Jessica L. Sneeden and Lawrence A. Loeb Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase Manel Camps and Lawrence A. Loeb Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast Ranga N. Venkatesan and Lawrence A. Loeb Autogene Selections Jijumon Chelliserrykattil and Andrew D. Ellington Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase Volker Sieber Proside: A Phage-Based Method for Selecting Thermostable Proteins Andreas Martin, Franz X. Schmid, and Volker Sieber Minimization of Proteins by Random Fragmentation and Selection Gary W. Rudgers and Timothy Palzkill Part II. Screens for Enzymes Evaluating a Screen and Analysis of Mutant Libraries Oriana Salazar and Lianhong Sun Screening Mutant Libraries in Saccharomyces cerevisiae Thomas Bulter, Volker Sieber, and Miguel Alcalde Solid-Phase Screening Using Digital Image Analysis Alexander V. Tobias and John M. Joern Screening for Thermostability Patrick C. Cirino and Radu Georgescu High-Throughput Screening of Mutant a-Amylase Libraries for Increased Activity at 129 DegreesC Holger Berk and Robert J. Lebbink High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450 Christopher R. Otey High-Throughput Screen for Aromatic Hydroxylation Christopher R. Otey and John M. Joern Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes Edgardo T. Farinas High-Throughput Screens Based on NAD(P)H Depletion Anton Glieder and Peter Meinhold High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases Radu Georgescu Screenfor Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase Lianhong Sun and Makoto Yagasaki Colorimetric Dehydrogenase Screen Based on NAD(P)H Generation Kimberly M. Mayer Colorimetric Assays for Screening Laccases Miguel Alcalde and Thomas Bulter pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity Karl E. Griswold A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase Huimin Zhao Detection of Aromatic a-Hydroxyketones with Tetrazolium Salts Michael Breuer and Bernhard Hauer Selection of Heat-Stable Clostridium cellulovorans Cellulases After In Vitro Recombination Koichiro Murashima and Roy H. Doi Screening and Selection Strategies for Disulfide Isomerase Activity Ronald Lafond, Xiaoming Zhan, and George Georgiou An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations Manfred T. Reetz Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes Manfred T. Reetz Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tRNA Synthetase Using Fluorescence-Activated Cell Sorting (FACS) Stephen W. Santoro and Peter G. Schultz Calmodulin-Tagged Phage and Two-Filter Sandwich Assays for the Identification of Enzymatic Activities Christian Heinis, Julian Bertschinger, and Dario Neri High-Throughput FACS Method for Directed Evolution of Substrate Specificity Mark J. Olsen, Jongsik Gam, Brent L. Iverson, and George Georgiou Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter Geoffrey S. Waldo Index