倍半萜
转录组
芸苔属
MYB公司
生物合成
化学
固相微萃取
气相色谱-质谱法
芳香
生物
生物化学
质谱法
色谱法
基因
植物
转录因子
基因表达
食品科学
作者
Haibin Wang,Chen Zong,Aimei Bai,Shuilin Yuan,Yan Li,Zhanghong Yu,Ruiping Tian,Xilin Hou,Xilin Hou,Ying Liu
标识
DOI:10.1016/j.fochms.2022.100129
摘要
Sesquiterpenes are important defensive secondary metabolites and aroma components. However, limited information is available on the mechanism of sesquiterpene formation and composition in the non-heading Chinese cabbage (NHCC) leaf. Therefore, headspace solid-phase microextraction/gas chromatography–mass spectrometry (HS-SPME/GC–MS) combined with transcriptome analysis was used to study the mechanism of volatile organic compound formation. A total of 26 volatile organic compounds were identified in two NHCC cultivars ‘SZQ’ and ‘XQC’ and their F1 hybrids. Among these, sesquiterpene β-caryophyllene was identified only in ‘XQC’ and F1. Five genes encoding caryophyllene synthase were identified. The candidate β-caryophyllene synthase genes BcTPSa11 and BcTPSa21 had high expression levels only in ‘XQC’ and F1. In addition, several transcription factors of MYB-related, MYB, bHLH, and AP2/ERF families were identified by co-expression, suggesting that they regulate β-caryophyllene biosynthesis. Our results provide a molecular basis for sesquiterpene biosynthesis as well as insights into the regulatory network of β-caryophyllene in NHCC.
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