细胞凋亡
糖基化
化学
下调和上调
细胞生物学
肿瘤坏死因子α
细胞内
炎症
癌细胞
程序性细胞死亡
癌症研究
生物
生物化学
癌症
免疫学
基因
遗传学
作者
Miyeon Jeon,Seung Un Seo,Seon Min Woo,Kyoung‐jin Min,Hee Sun Byun,Gang Min Hur,Sun Chul Kang,Taeg Kyu Kwon
出处
期刊:Biochimie
[Elsevier]
日期:2019-10-01
卷期号:165: 108-114
被引量:11
标识
DOI:10.1016/j.biochi.2019.07.015
摘要
Oridonin is a diterpenoid isolated from the Rabdosia rubescens and has multiple biological effects, such as anti-inflammation and anti-tumor activities. In present study, we revealed that the sensitizing effect of oridonin on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in several cancer cells, but not in normal cells. Oridonin enhanced death-signaling inducing complexes (DISC) formation and DR5 glycosylation without affecting expression of downstream intracellular apoptosis-related proteins. Oridonin upregulated peptidyl O-glycosyltransferase GALNT14 in a dose- and time-dependent manner. Knockdown of GALNT14 by siRNA and Endo H treatment reduced oridonin-induced DR5 glycosylation. Furthermore, treatment with inhibitor of glycosylation (benzyl-α-GalNAc) blocked oridonin plus TRAIL-induced apoptosis. Collectively, our results suggest that oridonin-induced DR5 glycosylation contributes to TRAIL-induced apoptotic cell death in cancer cells.
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