紫胶操纵子
报告基因
分子生物学
基因
β-半乳糖苷酶
生物
基因表达
遗传学
作者
Manfred Krämer,Robert Feil,Hannes Schmidt
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 29-45
标识
DOI:10.1007/978-1-0716-1008-4_2
摘要
Reporter mice transgenically expressing the bacterial (E. coli) lacZ gene encoding β-galactosidase (β-gal, EC 3.2.1.23) are a versatile and extensively used tool to study gene expression and cell lineage patterns. Enzymatic activity of the β-gal reporter can be effectively visualized at cellular resolution either histochemically using 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal) or by immunofluorescent detection using a β-gal-specific antibody. Here, we summarize protocols for the localization of β-gal expressing cells in whole embryos or organs as well as in histological tissue sections of lacZ reporter mice and discuss their limitations and common pitfalls.
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