化学
葡萄糖氧化酶
过氧化氢
过氧化氢酶
基质(水族馆)
酶
分子
生物物理学
生物化学
光化学
有机化学
生物
海洋学
地质学
作者
S. Sengupta,Krishna Kanti Dey,Hari S. Muddana,Tristan Tabouillot,Michael E. Ibele,Peter J. Butler,Ayusman Sen
摘要
Using fluorescence correlation spectroscopy, we show that the diffusive movements of catalase enzyme molecules increase in the presence of the substrate, hydrogen peroxide, in a concentration-dependent manner. Employing a microfluidic device to generate a substrate concentration gradient, we show that both catalase and urease enzyme molecules spread toward areas of higher substrate concentration, a form of chemotaxis at the molecular scale. Using glucose oxidase and glucose to generate a hydrogen peroxide gradient, we induce the migration of catalase toward glucose oxidase, thereby showing that chemically interconnected enzymes can be drawn together.
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