Plasmids of seven strains of bacteria identified into five genera were extracted with five different methods. The results showed that alkaline lysis method was suitable for the bacteria that could degrade organic compounds. Method used for pseudomonas was better for all the seven bacterial strains. In order to gain the primary information of plasmid of bacteria, the method used for pseudomonas is recommended as the preferential one; however, for further study different methods are needed.