压电1
肌腱
跳跃的
生物医学工程
机械转化
化学
剪应力
生物物理学
医学
材料科学
解剖
刚度
生物力学
离子通道
机械敏感通道
生物
细胞生物学
复合材料
生物化学
生理学
受体
作者
Fabian S. Passini,Patrick K. Jaeger,Aiman S. Saab,Shawn Hanlon,Nicole Angela Chittim,Matthias Arlt,Kim David Ferrari,Dominik Haenni,Sebastiano Caprara,Maja Bollhalder,Barbara Niederöst,Ágnes Horváth,Tobias Götschi,Shang Ma,Bettina Passini-Tall,Sandro F. Fucentese,Ulrich Blache,Unai Silván,Bruno Weber,Karin Grävare Silbernagel,Jess G. Snedeker
标识
DOI:10.1038/s41551-021-00716-x
摘要
Athletic performance relies on tendons, which enable movement by transferring forces from muscles to the skeleton. Yet, how load-bearing structures in tendons sense and adapt to physical demands is not understood. Here, by performing calcium (Ca2+) imaging in mechanically loaded tendon explants from rats and in primary tendon cells from rats and humans, we show that tenocytes detect mechanical forces through the mechanosensitive ion channel PIEZO1, which senses shear stresses induced by collagen-fibre sliding. Through tenocyte-targeted loss-of-function and gain-of-function experiments in rodents, we show that reduced PIEZO1 activity decreased tendon stiffness and that elevated PIEZO1 mechanosignalling increased tendon stiffness and strength, seemingly through upregulated collagen cross-linking. We also show that humans carrying the PIEZO1 E756del gain-of-function mutation display a 13.2% average increase in normalized jumping height, presumably due to a higher rate of force generation or to the release of a larger amount of stored elastic energy. Further understanding of the PIEZO1-mediated mechanoregulation of tendon stiffness should aid research on musculoskeletal medicine and on sports performance.
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