枯草芽孢杆菌
生物
基因座(遗传学)
基因
遗传学
DNA
基因表达
计算生物学
基因表达调控
细胞生物学
分子生物学
细菌
作者
Barbara Härtl,Wolfgang Wehrl,Thomas Wiegert,Georg Homuth,Wolfgang Schumann
标识
DOI:10.1128/jb.183.8.2696-2699.2001
摘要
The Bacillus subtilis lacA gene, coding for beta-galactosidase, has been explored as a new site able to accept DNA sequences from nonreplicating delivery vectors. Two such delivery expression vectors have been constructed and shown to be useful in obtaining regulated expression from the chromosomal location. In another experiment, it was shown that the integration of a regulatory gene at the lacA locus was able to control the expression of a transcriptional fusion at the amyE locus. These experiments demonstrate that both integration sites can be used simultaneously to obtain regulated expression of desired genes.
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