生物
基因
互补DNA
水稻
打开阅读框
真核翻译
遗传学
五素未翻译区
分子生物学
基因表达
非翻译区
编码区
信使核糖核酸
cDNA文库
作者
Ji Huang,Jianfei Wang,Sheng-Ping Qiu,Hongsheng Zhang
出处
期刊:Dna Sequence
日期:2004-02-01
卷期号:15 (1): 39-43
被引量:5
标识
DOI:10.1080/1042517032000160189
摘要
The eukaryotic initiation factor 1A(eIF1A) is essential for transferring of the initiator Met-tRNA to 40S ribosomal subunits to form the 40S pre-initiation complex. In present study, we describe the cloning and characterization of two eIF1A genes from rice, which were designated as Oryza sativa eukaryotic initiation factor 1A genes OseIF1A-1, OseIF1A-2, respectively. Both rice eIF1As shared high identities in amino acids with eIF1A proteins from other eukaryotes. The mRNA expression analysis revealed that OseIF1A-2 mRNA was much more accumulated than OseIF1A-1 in all tissues but each gene is expressed in root, stem, leaf and flowering spike in high and nearly equal level, and in immature spike in lower level. These results, together with their different location in unrooted phylogenetic tree inferred from amino acid sequences of all known eIF1As, suggested that there are two types of eIF1A genes with different function or different regulation in rice.
科研通智能强力驱动
Strongly Powered by AbleSci AI