杀虫剂
毒理
DNA损伤
线粒体DNA
危害
生物
化学
生态学
DNA
遗传学
基因
作者
Chao Zeng,Qing Cheng,Wei Li,Shu Yan,Kaiye Li,Chensheng Lu
出处
期刊:Environmental Science and Technology Letters
[American Chemical Society]
日期:2024-02-14
卷期号:11 (3): 195-200
被引量:1
标识
DOI:10.1021/acs.estlett.3c00914
摘要
The increasing popularity of seed treatment applications in agriculture may leave unintended hazards to soil biota, such as earthworms. The objective of this study was to explore mitochondrial DNA toxicity resulting from sublethal exposures to systemic pesticides, including four neonicotinoid insecticides (neonics), as well as coexposure to difenoconazole (DIF), a triazole fungicide, in earthworms (Eisenia fetida) in vivo. Earthworms were exposed under dose regimes resembling label recommendation and levels left in soil post seed treatment application for 30 days in an earthworm breeding facility. Mitochondrial DNA copy numbers (mtDNAcn) in earthworms were determined by using the 2–ΔCt algorithm. Earthworms' body weights were recorded before and after the exposure period. We found a highly significant increase of mtDNAcn in earthworms across all exposure groups (ANOVA, p < 0.001), either under a single neonic or combined with DIF exposure. Coupled with mtDNA toxicity, earthworms in the treatment groups gained significantly less weight than control earthworms (ANOVA, p < 0.001). We concluded that systemic pesticides, both neonics and DIF, posed mtDNA toxicity as measured by mtDNAcn, in earthworms.
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