质外体
细胞外小泡
拟南芥
拟南芥
十字花科
小泡
细胞外
生物
植物
生物物理学
细胞生物学
细胞壁
生物化学
基因
膜
突变体
作者
Yuan Liu,Sherry Y. Wu,Yeonjong Koo,Yang An,Yun Dai,Htet Khant,Samantha R. Osman,Mamur A. Chowdhury,Haichao Wei,Yang Li,Karem A. Court,Elaine Hwang,Yunfei Wen,Santosh K. Dasari,Michael Nguyen,E. Chia Cheng Tang,E. Wassim Chehab,Natalia de Val,Janet Braam,Anil K. Sood
标识
DOI:10.1016/j.nano.2020.102271
摘要
Mammalian small extracellular vesicles (sEVs) can deliver diverse molecules to target cells. However, they are difficult to obtain in large quantities and can activate host immune responses. Plant-derived vesicles may help to overcome these challenges. We optimized isolation methods for two types of plant vesicles, nanovesicles from disrupted leaf and sEVs from the extracellular apoplastic space of Arabidopsis thaliana. Both preparations yielded intact vesicles of uniform size, and a mean membrane charge of approximately −25 mV. We also demonstrated applicability of these preparative methods using Brassicaceae vegetables. Proteomic analysis of a subset of vesicles with a density of 1.1-1.19 g mL−1 sheds light on the likely cellular origin and complexity of the vesicles. Both leaf nanovesicles and sEVs were taken up by cancer cells, with sEVs showing an approximately three-fold higher efficiency compared to leaf nanovesicles. These results support the potential of plant-derived vesicles as vehicles for therapeutic delivery.
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