DNA
细胞内
计算机科学
生物分子
计算生物学
小RNA
微流控
化学
分子生物学
纳米技术
生物
生物物理学
细胞生物学
材料科学
生物化学
基因
作者
Min Bai,Feng Chen,Xiaowen Cao,Yue Zhao,Jianru Xue,Xu Yu,Chunhai Fan,Yongxi Zhao
标识
DOI:10.1002/anie.202001598
摘要
Abstract Tumor progressions such as metastasis are complicated events that involve abnormal expression of different miRNAs and enzymes. Monitoring these biomolecules in live cells with computational DNA nanotechnology may enable discrimination of tumor progression via digital outputs. Herein, we report intracellular entropy‐driven multivalent DNA circuits to implement multi‐bit computing for simultaneous analysis of intracellular telomerase and microRNAs including miR‐21 and miR‐31. These three biomolecules can trigger respective DNA strand displacement recycling reactions for signal amplification. They are visualized by fluorescence imaging, and their signal outputs are encoded as multi‐bit binary codes for different cell types. The results can discriminate non‐tumorigenic, malignant and metastatic breast cells as well as respective tumors. This DNA computing circuit is further performed in a microfluidic chip to differentiate rare co‐cultured cells, which holds a potential for the analysis of clinical samples.
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