Research on isolation of βasaron compound from Sweet Flag rhizome Acorus calamus L.. The aims of this research are to isolate and to identify mayor compound βasaron from Sweet Flag rhizome Acorus calamus L.. Sample from 150 gram dried rhizome, mascerated using n-hexane producing 4,22 grams thick extract.Isolation of n-hexane extract Sweet Flag rhizome Acorus calamus L. carried out by chromatotron methods using a comparison of eluen n-hexane : etil acetat (9:1).The isolate identified by UV-Vis spectroscopy, IR spectroscopy, and Gas Chromatography Mass Spectro.From the data result show that isolate is β-asaron.