Apoptosis in Human cervix cancer Hela cells induced by two group concentrations of proteasome inhibitor MG132

To investigate the effect of different concentration proteasome inhibitor (MG132) on the apoptosis of human cervix cancer Hela cells,Hela cells was cultured with different concentrations of proteasome inhibitor (MG132),and the cell viability was determined by MTT assay. The cell apoptosis rate was determined by Annexin Ⅴ/PI staining and flow cytometry. Caspase-3 activity change was tested by Western blotting and ELISA Reader. The results indicate that high concentration of proteasome inhibitor (MG132) is more significant in injury and apoptosis of Hela cells than low concentration one. The different concentration of proteasome inhibitor (MG132) is obvious in inducing apoptosis of hela cells.