Study of the Human Bone Morphogenetic Protein-2 Recombinant Adenovirus in the Treatment of Bone Defect

Objective To construct the recombinant adenovirus of human bone morphogenetic protein-2 and to detect its expression and activity in tissues. Methods The cDNA coding for the full length BMP2 was amplified, purified, and inserted into the vector PGEM-T-Easy. The recombinant virus was constructed using the DNA/calcium phosphate precipitate containing PGEM-T-BMP2 and PJM17, 30 SD rats were divided into two groups randomly and 50 μl purified recombinant adenovirus was injected into the calf muscle of the rats.The expression of BMP gene in muscle tissue was measured and the changes of ALP staining and X-ray examination were compared. Results Significant activity of bone induction was found in the treatment group with the recombinant adenovirus of hBMP2, compared with the normal control group. The level of ALP activity in the muscular tissues of the treatment group was higher than that in the control group. Conclusion The recombinant AdBMP2 was constructed and the hBMP2 is functionally active as demonstrated by the induction of ALP activity in the muscular tissues of animal model.