Inhibition of SAHA on HeLa and p53 Knock-down HeLa-E3 Cells Proliferation

Objective To investigate the effect of SAHA(suberoylanilide hydroxamic acid)on p53 wild type HeLa cells and p53 knock-down HeLa-E3 cells.Methods The cell's proliferation were determined by MTT after 24,48 and 72 h incubation;the cell cycle distribution and cell death were detected by flow cytometry and typan-blue assay,respectively.Results SAHA significantly suppressed the in vitro proliferation of HeLa and HeLa-E3 cells in a time and dose dependent manner.G2/M arrest and cell death of HeLa cells were also elevated by SAHA in 24 h,while HeLa cells were more sensitive to SAHA than HeLa-E3 cells.Conclusion The results show that the sensitivity of tumor cells to SAHA were influenced by p53.