Screend Target Gene GALK1 of Transcription Factor AP-2 Alpha by Chromatin Immunoprecipitation Assay

In the post-genomic era, identifying and characterizing various DNA-protein interactions are a major challenge in the research of gene transcriptional regulation. Nowadays, in comparison with other methods, chromatin immunoprecipitation assay (ChIP) is ideally suited for studying DNA-protein interactions in vivo. In this study, by utilizing the chromatin immunoprecipitation assay, the unknown target gene GALK1 of transcription factor AP-2 alpha has been screened, and we validated the results with Luciferase assay and RT-PCR. With the new discovery, we can study further about the function of the transcription factor AP-2 alpha.