Glycopeptide Analysis Using LC/MS and LC/MSn Site-Specific Glycosylation Analysis of a Glycoprotein

A glycoprotein consists of heterogeneous molecules attached to diverse oligosaccharides at multiple glycosylation sites. Glycosylation analysis at each glycosylation site is important for understanding the function of carbohydrate moiety in glycoproteins. MS of glycopeptides is a commonly used method for the analyses of glycosylation sites and site-specific glycosylations (1–4). Since glycopeptide ions sometimes fail to be acquired by MS in the presence of excess peptides due to their lower ionization efficiency, separation or enrichment of glycopeptides is crucial for the MS of glycopeptides to be performed. LC/MS that allows for separation of glycopeptides from a mixture of peptides and acquisition of their mass spectra is one of the most effective means in use for the site-specific glycosylation analysis of glycoproteins. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.