Digoxigenin (DIG) Labeling of IgG Antibody

Digoxigenin (DIG) is a plant steroid (390 Da) that can be used as a small, stable label of immunoglobulin G (IgG) molecules. It is a valuable alternative to biotin as the biotin-streptavidin system can sometimes give high backgrounds due, for example, to the presence of biotin-containing enzymes in the sample. There is a range of commercially available mouse and sheep anti-DIG Fab antibody fragments labeled with various enzymes and fluorescent molecules for the detection of the DIG-labeled IgG antibody in many applications ( 1 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.