Portable multichannel immunoassay of coxsackievirus A6 using a coordination-engineered iridium-doped ZIF-8 nanozyme

. In spiked human serum samples, satisfactory recoveries of 95.85-101.00% for ECL, 97.13-101.15% for colorimetry, and 93.48-111.45% for PL were obtained, with relative standard deviations of 3.85-4.79%, 1.32-2.67%, and 4.42-5.73%, respectively. This work not only provides insight into the coordination-dependent catalytic enhancement of iridium-doped MOF-based nanozymes, but also offers a reliable multichannel immunoassay platform for virus analysis.