Opportunities presented by zebrafish larval models to study neutrophil function in tissues

Appropriate neutrophil function is essential for innate immune defence and to avoid inflammatory pathology. Neutrophils can adapt their responses according to their environment and recently, the existence of multiple distinct neutrophil populations has been confirmed in both health and disease. However, the study of neutrophil functions in their tissue environment has remained challenging, and for instance, the relationship between neutrophil maturity and function is not fully understood. Many neutrophil morphological and functional features are highly conserved between mammals and non-mammalian vertebrates. This enables the use of the transparent and genetically tractable zebrafish larvae to study neutrophil biology. We review data on the development and function of zebrafish larval neutrophils and advances zebrafish have brought to studies of neutrophil biology. In addition, we discuss opportunities and aspects to be considered when using the larval zebrafish model to further enhance our understanding of neutrophil function in health and disease.