ANALYSIS OF GENOTYPES AND SUBTYPES OF HEPATITIS B VIRUS IN TWO COUNTIES OF SICHUAN

[Objective] To investigate genotype and subtype of hepatitis B virus in 2 counties(districts) of Sichuan province.[Methods] Virus DNA were extracted from samples both positive for HBsAg and HBeAg.After polymerase reaction(PCR) and sequencing of partial S gene,genotypes and subtypes were analysized by phylogenetic analysis and deduced amino acid sequences,respectively.[Results] Of 49 samples,those with w subdeterminant and those with r subdeterminant be-longed exclusively to genotype B and genotype C,respectively;31 samples were genotype B/subtype adw2,1 was genotype B/subtype ayw1,1 was genotype B/subtype adw3,14 were genotype C /subtype adrq+,and 2 were genotype C /subtype ayr.[Conclusion] Genotype B /subtype adw2 and genotype C/subtype adrq+ accounted for most proportion,and genotype B/sub-type adw2 predominated in 2 counties(districts);rare genotype B/subtype adw3 also existed.