The Experimental Study of the Anti-SARS-CoV Effect of Interferon-α Interferon-γ and Combination of Interferon-α and Interferon-γ in Vitro

Aim To evaluate the anti-SARS-CoV effect of interferon-α,interferon-γ and the combination of interferon-α and interferon-γ at 5 different ratios in vitro.Methods The antiviral activity of interferon-αand interferon-γ was determined by MTT colorimetry.Results The TCID50 of SARS-CoV was 10-7.Interferon-α and interferon-γ had anti-SARS-CoV activity in vitro.The anti-SARS-CoV effect of Interferon-α was better than that of interferon-γ.And the anti-SARS-CoV effect of the combination of Interferon-α and interferon-γ was better than that of any single drug.Conclusion Interferon-α,interferon-γ and the combination of interferon-α and interferon-γ all have anti-SARS-CoV activity in vitro.This study has provided experimental evidence for the clinical use of antiviral treatment of the SARS-CoV infection.