One important method of chromatographic analysis technique as separation was one of the most vibrant areas in analytical chemistry,which can isolate compounds with very small performance difference,high efficiency and high sensitivity for protein separation and analysis research was one of the hotspot in the field of life science research.The research progress of several common and the recently emerged chromatography technology were elaborated.The recent applications and development of modern chromatographic technique in protein separation and analysis were introduced concisely.