Haemolysis of various mammalian erythrocytes in sodium chloride, glucose and phosphate-buffer solutions

Mouse, rat, rabbit, hamster, cow, pig, sheep, guineapig, dog and human erythrocytes were studied. A 0·9% or stronger solution of sodium chloride completely prevented haemolysis; sheep and pig erythrocytes appeared the more fragile, while human and dog erythrocytes were not haemolized in concentrations of 0·4% or more. Haemolysis of human, rabbit, cow, hamster, guineapig, pig and sheep erythocytes was not observed in solutions of 0·4% or more of glucose. Except for sheep, human and dog erythrocytes, haemolysis was depressed in rate but not completely prevented by phosphate-buffer solution of pH 7·0.