线性范围
安培法
微分脉冲伏安法
循环伏安法
材料科学
电化学气体传感器
纳米化学
介电谱
核化学
分析物
作者
Rita C. Alves,Filipa B. Pimentel,Henri P. A. Nouws,Raquel C.B. Marques,María Begoña González-García,M. Beatriz P.P. Oliveira,Cristina Delerue-Matos
标识
DOI:10.1016/j.bios.2014.08.026
摘要
A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter = 32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody-antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD 96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.
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