Ratiometric fluorescence sensing with logical operation: Theory, design and applications

The construction of ratiometric fluorescence sensing logic systems has gradually become a hot topic in fluorescence analysis, due to the multi-target analysis potential of logic operations and the high specificity and selectivity of ratiometric fluorescence sensing. In this paper, the basic principles of various logic functions implemented in ratiometric fluorescence detection are discussed in the context of sensing mechanisms, and the strategies for constructing logic systems in different ratiometric fluorescence sensing application areas are summarized. Although there are limitations such as cumbersome operations and complicated experiments, ratiometric fluorescence sensing logic circuits that combine the visualization of logic operations and the accuracy of ratiometric fluorescence are still worthy of in-depth study. This review may be useful for researchers interested in the construction of logic operations based on ratiometric fluorescence sensing applications.