The unique properties of two-dimensional molybdenum disulfide (2D MoS 2 ) have so far led to immense research regarding this material’s fundamentals, applications, and, more recently, its potential for biosensing. 2D MoS 2 has properties that make it of great interest for developing biosensors. These properties include large surface area, tunable energy band diagrams, a comparatively high electron mobility, photoluminescence, liquid media stability, relatively low toxicity, and intercalatable morphologies. In this Review, the current progress on 2D MoS 2 based biosensors is presented and the prospects for future possibilities of expanding its applications for a variety of biosensing applications are discussed.