Possible mechanisim of β-Amyloid peptide 25-35-induced abnormal cell cycle in PC 12 cells

Objective To study the effect ofAβ25-35 on PC12 cell cycle and expression of cell cycle related genes. Methods After treated with 25 μmol/L Aβ25-35, cell cycle, apoptosis, and expression of p21, Cyclin D1, pRb and E2F1 were detected by flow cytometry, electrophoresis, RT-PCR and Western-blot in PC12 cells, respectively. Results Percentage of S phase cells increased remarkably at 8 h, but decreased at 16 h and 24 h after Aβ25-35 treatment (P0.01). Cell apoptosis elevated obviously after 16 h (P0.01). Expression of p21 decreased, but that of Cyclin D1, pRb and E2F1 increased after 4 h of Aβ25-35 treatment. Conclusion Aβ25-35 can induce the abnormal cycle and apoptosis of synchronized PC12 cells, which is likely related to downexpression of p21 and overexpression of Cyclin D1, pRb and E2F1.